NET1 (Neuroepithelial Cell-transforming Gene 1 Protein, Proto-oncogene p65 Net1, Rho Guanine Nucleotide Exchange Factor 8, ARHGEF8)

Antibodies / Antibodies-GTPase, Rab, Ras, Rho Proteins

-20°C

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Synthetic peptide corresponding to C-DKLEYLDEKQRDPR, from human NET1, at the internal region of the protein (NP_005854.2, NP_001040625.1).

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:16, 000
Immunohistochemistry (Paraffin): 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.