Neurofilament M,H 160-200kD (NF-M+H) (MaxLight 490)

Mab

MaxLight 490

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.

Adult rat neurofilament

MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73, 000.

Neurofilaments are intermediate (10–12nm) filaments located specifically in neurons. There are three classes of neurofilaments: NF-L (68kD), NF-M (160kD), and NF-H (200kD). The neurofilaments are long helical proteins, which polymerize to form a rigid cytoskeleton in the neuron. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, phosphorylation likely mediates neurofilament dynamics. However, how O-linked N-acetylglucosamine residues function to modify neurofilament function remains unclear.

Applications:
Suitable for use in FLISA, Immunohistochemistry, Immunoprecipitation, and Western Blot. Other applications not tested.

Recommended Dilution:
FLISA: 0.1–0.5ug/ml
Immunohistochemistry (Bouin’s and alcohol-fixed, paraffin-embedded sections and frozen sections): 5-10ug/ml. To stain, incubate 30–60 minutes at RT or overnight at 4°C.
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 2-5ug
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.