Neurofilament H, 180-220kD (NF-H)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Rat brain protein preparation

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160 and 200kD) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70kD NF-L protein can self-assemble into a filamentous structure, however the 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 5ug/ml. Optimal staining with citrate buffer, pH 6.0.
Immunohistochemistry: Acetone-fixed frozen sections
Western Blot: 1:500 detected Neurofilament H on 10ug of mouse brain membrance lysates.
Optimal dilutions to be determined by researcher.

Positive Control:
Brain; Cultured neurons

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.