Neurofilament, Heavy Chain (NF-H)

Pab

IgY

-20°C

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% sodium azide.

Purified neurofilament protein (200kD) isolated from bovine brains. Species sequence homology: Human, mouse.

Human Neurofilament Heavy Chain (NF-H) is a 115.378kD protein (1072aa) that forms part of the neuronal cytoskeleton. NF-H is physically associated with 10nm intermediate filaments, and is said to be one of the “neurofilament triplet” proteins, although it probably serves more to stabilize the cytoskeleton, rather than to be an integral structural component. NF-H immunoreactivity is found in neuronal somata, dendrites and axons.

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:2000:1:5000 using 2% paraformaldehyde tissues
Immunocytochemistry: 1:2000:1:5000 using 2% paraformaldehyde cells
Western Blot: 1:5000-1:10, 000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906:IgY, Chicken Pab Rb x Ch
I1906-10:IgY, Chicken (HRP) Rb x Ch
I1906-12:IgY, Chicken (FITC) Rb x Ch
I1906-14:IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.