NF-H (Neurofilament heavy polypeptide, Neurofilament triplet H protein, 200kD, NEFH, KIAA0845, NFH)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

Partial recombinant human NF-H protein (263-364) with a GST tag.

Neurofilaments contain three intermediate filament proteins: L, M and H, which are involved in the maintenance of the neuronal caliber. NF-H has an important function in mature axons. NF-H is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Defects in NF-H are a cause of amyotrophic lateral sclerosis (ALS) which is a neurodegenerative disorder affecting upper and lower motor neurons and results in fatal paralysis.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.