P4HA1 (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1)

Antibodies / Antibodies-Enzymes, Oxidase

-20°C

Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Synthetic peptide corresponding to C-DFARKDEPDAFKE, from P4HA1, at the internal region of the protein (NP_000908.2, NP_001017962.1).

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:32, 000
Western Blot: 0.1ug/ml, observed in lysates of cell lines HEK293, A431 and NIH3T3 on ~60kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.