EP300, phosphorylated (Ser89) (Histone Acetyltransferase p300, E1A-associated Protein p300, p300 HAT)

Antibodies / Antibodies-Enzymes, Acetyltransferase

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser89 of human EP300 (KLH).

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

Applications:
Suitable for use in ELISA, Western Blot, Dot Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.