PAEL Receptor (GPR37, PAELR, G-protein Coupled Receptor 37, ETBR-LP-1, Endothelin B Receptor-like Protein 1, Parkin-associated Endothelin Receptor-like Receptor)

Antibodies / Antibodies-G Protein Receptors

-20°C

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

NS0-derived transfected with human GPR37 (UniProt #O15354)

GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 613aa, ~55kD orphan 7-transmembrane receptor. It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus. It is a substrate of the E3 ubiquitin ligase, parkin, which is upregulated during endoplasmic reticulum stress. In a juvenile form of Parkinson’s disease, GPR37 accumulates, contributing to stress-induced neuronal cell death. The extracellular portions of human and mouse GPR37 share 68% aa identity.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 2.5ug/10e6 cells A172 human glioblastoma cell line fixed paraformaldehyde and permeabilized with saponin
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.