Parkin (RING-type E3 ubiquitin-protein ligase)

Antibodies / Antibodies-Ubiquitin Ligases

-20°C

Supplied as a liquid, 0.09% sodium azide.

Full length recombinant human Parkin

Parkin is a ubiquitin-protein isopeptide ligase (E3) involved in ubiquitin/proteasome-mediated protein degradation. Parkin may protect cells from premature death by targeting misfolded or damaged proteins for degradation via the ubiquitin proteasome pathway. Mutations in the parkin gene cause a loss-of-function and/or alter protein levels of parkin. The loss of functional parkin is the most common single cause of autosomal recessive Parkinson’s disease.1-2 Parkin expression is neuron-specific and developmentally regulated, and thus, has a role in neuronal maturation. Parkin may also play roles in cell cycle regulation and tumor suppression.3

Applications:
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Immunoprecipitation: 100ul per test
Western Blot: 1:20-1:100
Immunofluorescence: 1:20-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.