Plexin C1 (CD232, Virus-Encoded Semaphorin Protein Receptor, VESPR)

Antibodies / Antibodies-Plexin, Semaphorin

-20°C

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

Recombinant corresponding to aa49-161 from human Plexin C1 expressed in E. coli (O60486).

Plexin C1 is a 210kD member of the plexin-C subfamily, plexin family of proteins. It is a type I transmembrane glycoprotein that is found on monocytes, dendritic cells and neutrophils. Plexin C1 binds Sema7A, and its viral counterpart, vaccinia semaphorin A39R. Ligation induces actin cytoskeletal rearrangement and cell immobilization. Mature human Plexin C1 is 1534aa in length. It contains a 910aa extracellular domain (ECD) and a 603aa cytoplasmic region. The ECD contains one Sema-domain (aa49-440), two PSI domains (aa454-605) and two IPT regions (aa664-842). Over aa49-161, human Plexin C1 shares 92aa identity with mouse Plexin C1.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.