PSD95, Postsynaptic Density Protein 95 (PSD-95, PSD95, Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90)

Mab

IgG2a, k

-20°C

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Recombinant protein corresponding to human PSD95.

PSD95 (postsynaptic density 95) represent a class of anchoring proteins which serve to localize various neuronal ion channels to post-synaptic densities. PSD95 and related proteins contain PDZ domains, which interact with NMDA receptors and voltage-gated K+ channels via a ET/SXV motif in the cytoplasmic carboxyl terminus of the receptor channels.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5ug/ml detects PSD95 on 10ug of rat brain tissue lysate. Rat brain lysate was probed with N5380-25. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunohistochemistry: 1:500 detects PSD95 in rat cortex tissue.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain tissue lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.