UCHL1/PGP9.5 (PGP9.5,PGP-9.5,Gracile Axonal Dystrophy,Neuron Cytoplasmic Protein 9.5,Parkinson Disease 5,Park5,Ubiquitin Carboxyl Terminal Esterase L1,Ubiquitin C-terminal Esterase L1,Ubiquitin Carboxyl Terminal Hydrolas

Pab

IgY

-20°C

Supplied as a liquid, 0.02% sodium azide.

Recombinant corresponding to full length human Protein Gene Product 9.5 purified from E. coli.

PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Protein gene product (PGP9.5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. The protein which has a molecular weight of 27kD was first identified by high resolution two dimensional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibers at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.

Applications:
Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Not recommended for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IC): 1:500-1:1000
Western Blot: 1:2000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.