SARS M Protein (Severe Acute Respiratory Syndrome)

Antibodies / Antibodies-Infectious Disease SARS

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

Raised against a synthetic peptide corresponding to amino acids at the carboxy-terminus of the SARS M protein (Genbank accession no. P59596)

A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2). Coronaviruses are a major cause of upper respiratory diseases in humans (3). The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. The M protein (Membrane protein, Matrix protein) is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein and the nucleocapsid protein (4-5).

Applications:
Can be used for the detection of SARS M protein in ELISA. It will detect 10ng of free peptide at 1mg/ml.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.