SARS, Spike Protein (Severe Acute Respiratory Syndrome, SARS-CoV, SARS Associated Coronavirus)

Antibodies / Antibodies-Infectious Disease SARS

-20°C

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

Synthetic peptides corresponding to amino acids 288-303 of the putative SARS Spike glycoprotein (Genbank accession no. NP_828851)..

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kD protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Applications:
Suitable for the detection of SARS Spike protein in western blot analysis and ELISA. The antibody was tested on a cell line transfected with a partial-length cDNA coding for the N-terminal portion of SARS Spike protein with a predicted molecular weight of 68kD. Western blot analysis of SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using antibody at 0.1ug/ml.

Recommended Dilution:
Western blot: 0.1-1.0ug/ml
ELISA: 0.1-1.0ug/ml

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.