SARS, Spike Protein (Severe Acute Respiratory Syndrome, SARS-CoV, SARS Associated Coronavirus)

Antibodies / Antibodies-Infectious Disease SARS

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

Raised against a synthetic peptide corresponding to amino acids 656-672 of the SARS Spike glycoprotein (Genbank accession no. P59594)

A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2). Coronaviruses are a major cause of upper respiratory diseases in humans (3). The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139kD protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2 (4).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: Detects 10ng of free peptide at 1ug/ml.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.