STAT3, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063)

Antibodies / Antibodies-Transcription Factors, STAT

-20°C

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Synthetic phosphopeptide corresponding to human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).

The transcription factor, signal transducer and activator of transcription-3 (STAT-3) is the most pleiotropic member of the signal transducer and activator of transcription (STAT) family of transcription factors and mediates pivotal responses for the cytokine family. The mouse STAT3 gene contains 24 exons and spans 30kb. The translation initiation codon is in exon 2, and the stop codon is in exon 24. STAT3 is mapped to 17q21, it contributes to various physiological processes. Hepatic STAT-3 signaling is thus essential for normal glucose homeostasis and may provide new therapeutic targets for diabetes mellitus.

Applications:
Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:100-1:200
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.