TACE (Disintegrin and Metalloproteinase Domain-containing Protein 17, TNF-alpha-converting Enzyme, TNF-alpha Convertase, Snake Venom-like Protease, CD156b Antigen, CSVP, TNF-a Converting Enzyme, ADAM17)

Antibodies / Antibodies-Matrix Metalloproteinases

-20°C

Supplied as a liquid in PBS, pH 7.5.

Human TACE pure protein (Extracellular domain, aa1-671).

b-amyloid (Ab) deposition in the brain is the hallmark of Alzheimer's Disease (AD). To initiate Ab formation, b-secretase cleaves APP at the N-terminus of Ab to release APPsb (~100kD soluble NT-fragment), and C99, a 12kD CT membrane fragment. Alternatively, a-secretase cleaves within the Ab to prevent the formation of Ab. Cleavage by a-secretase produces a soluble Nterminal fragment, APPsa, and a 10kD membrane C-terminal fragment, C83. Both C99 and C83 can be further cleaved by gsecretase releasing Ab and a nonpathogenic p3 peptide, respectively. Recently TACE, a member of the ADAM family (A Disintegrin And Metalloprotease family) protease has been shown to play a central role in a regulated cleavage of human APP. Inhibition of TACE affects both APP secretion and Ab formation in cultured cells. Membrane-bound TNF-a, like APP, is transmembrane protein that can undergo TACE-mediated proteolysis to release the extracellular domain as soluble TNF-a. TACE contain an autoinhibitory domain that must be removed for activity, a proteolytic domain, a disintegrin domain, a cysteine-rich domain, and a Transmembrane domain.

Applications:
Suitable for use in ELISA and Flow Cytometry. Though not tested, Western Blot may potentially be used as an application. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10, 000-1:100, 000
Flow Cytometry: 0.5-1ug/10e6 cells (blood lymphocytes or THP-1 cells).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.