TFCP2L1 (Transcription Factor CP2-like 1, CP2-related Transcriptional Repressor 1, CP2-related Transcriptional Repressor-1, CRTR1, CRTR-1, LBP9, LBP-9, Transcription Factor CP2-like Protein 1, Transcription Factor LBP-9)

Antibodies / Antibodies-Transcription Factors

-20°C

Supplied as a lyophilized powder in PBS, pH 7.2, with 5% trehalose. Reconstitute with 500ul sterile PBS

E. coli-derived rhTFCP2L1 (aa 1-180).

TFCP2L1 (Transcription factor CP2-like protein # 1; also LBP-9) is a 54kD (predicted) member of the CP2 subfamily, grh/CP2 family of proteins. It is expressed in placental synctiotrophoblast cells, and regulates the production of CYP11A1/P450scc. P450scc is a cholesterol cleavage enzyme that generates pregnenolone, a necessary intermediate in the formation of progesterone. TFCP2L1 both positively and negatively regulates P450scc synthesis through homodimerization and heterodimerization with LBP-1b. Human TFCP2L1 is 479 amino acids (aa) in length. It contains a transcriptional repressor region (aa 100-200) and a dimerization domain (aa 300-479). There is one splice variant that shows a deletion of aa 400-464. Human TFCP2L1 is 73% aa identical to human TFCP2, and 93% aa identical to mouse CRTR-1, a closely related molecule that may not represent the actual mouse ortholog to TFCP2L1.

Applications:
Suitable for ELISA and Western Blot.Other Applcations not tested.

Recommended Dilution:
ELISA:
Western Blot:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.