Toll Like Receptor 2, NT (TLR2, CD282) (APC)

Antibodies / Antibodies-Receptors

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Peptide corresponding to an 14 amino acid sequence near the amino terminus of human TLR2.

Toll Like Receptor 2 (TLR2) is a 90kD type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. TLR2 is also expressed in the spleen, bone marrow and lymph nodes. TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

Applications:
Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: detected a band of ~100kD in A-20 cell lysates.
Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer, pH 6.0 is recommended.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human spleen

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 

Note: Applications are based on unconjugated antibody.