DR5 (Apo2, Trail-R2, TRAIL Receptor2, Trick2, Killer, Death Receptor5, CD262, KILLER, KILLER/DR5, TNFRSF10B, TRANCE-R, TRICKB, Tumor Necrosis Factor Receptor Superfamily, Member 10b, ZTNFR9)

Antibodies / Antibodies-Apoptosis

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.

Synthetic peptide (NH2-APQQKRSSPSEGLCPPGHHISEDGRDC) corresponding to the extracellular domain sequence of human TRAIL-R2.

CD262, otherwise known as DR5/TRAIL-R2, is a broadly expressed death domain containing receptor and member of the TNF-receptor superfamily, which acts as a mediator for CD253/TRAIL induced apoptosis in a variety of tumor and transformed cells, but not in normal cells.
Expression of CD262 is regulated by tumor suppressor p53, and CD262 is highly expressed by tumor cell lines. Studies suggest that the death domain containing adaptor protein FADD, is required for CD262-mediated apoptosis, and that FADD recruits caspase-8 to the activated receptor.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA (indirect): 1:75, 000
Western Blot: 1:500
Immunohistochemistry (paraffin): 1:150
Flow Cytometry: 1ul (~10e6 cells/100ul)
Optimal dilutions to be determined by the researcher.

Positive Control:
Spleen

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.