Tsp23 (Transcript Similar p23)

Antibodies / Antibodies-Enzymes, Synthetase

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.

Recombinant corresponding to human Tsp23.

p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the hsp90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25kD) with a simple structure (2, 3). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated hsp90 binding of client proteins (8). Since many hsp90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). Hsp90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology. Specifically TSP23 (transcript similar p23) displays 44% and 17% amino acid identity with p23 and Sba1p respectively (10).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml was sufficient for detection of tsp23 in 20ug of transiently transfected Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.