TRIAD3A (E3 Ubiquitin Ligase TRIAD3A,E3 ubiquitin-protein ligase RNF216,RING finger protein 216,Ubiquitin-conjugating enzyme 7-interacting protein 1,UbcM4-interacting protein 83,Triad domain-containing protein 3,Rnf216,T

Pab

MaxLight 550

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

A 15aa peptide located near mouse TRIAD3A amino-terminus.

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150, 000.

TRIAD3A is a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response.

Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.