Tubulin, beta (Beta Tubulin, Tubulin beta Chain, Tubulin beta Polypeptide, TUBB, TUBB Protein, DAQB-47P19.9-001, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB 1, TUBB1, Tubulin beta-5 Chain, TUBB 5, TUBB5, XXbac-BCX111D4.2

Mab

IgG2b, k

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Recombinant corresponding to aa1-455 of human beta-Tubulin, expressed in E.coli.

Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Applications:
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:1000-1:5000
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Hybridoma:
F0 myeloma cells with spleen cells from BALB/c mice

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.