Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Antibodies / Antibodies-Tubulin

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T9155-50X.

Native chick brain microtubules. Epitope: aa426-450. Cellular Localization: Cytoplasmic.

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.

Applications:
Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G.
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:400 for 20 minutes at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
LS174T, MAD109 cells. Skin or lung.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.