Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Antibodies / Antibodies-Tubulin

-20°C

Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute in sterile ddH2O, PBS.

Microtubules from chicken embryo brain.

Alpha-tubulin (b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55kD. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451aa protein that is 100% identical to the rat homolog and differs by only 2 and 3aa from the pig and chicken homologs, respectively.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Immunohistochemistry (paraffin): 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.