Uncoupling Protein 2 (UCP2, SLC25A8, UCPH)

Antibodies / Antibodies-Obesity Proteins

-20°C

Supplied as a liquid in PBS, 0.1% BSA.

Synthetic peptide corresponding to a 13aa sequence between the 3rd and 4th transmembrane domain of human UCP2 conjugated with KLH. Species Sequence Homology: rat and mouse; 100%, canine, bovine and porcine; 92%

White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-5) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic. Mouse/rat UCP2 is a 309aa (human 309aa, chromosome 7; ~95% homology) mitochondrial uncoupling protein (1). It is only 59% homologous with UCP1 found in brown adipose tissues. UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart and muscle). UCP2 may play a critical role in energy balance, body weight and thermoregulation.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10, 000-1:100, 000 using 50-100ng of control peptide/well.
Western Blot: 1-10ug/ml (ECL)
Immunohistochemistry: 2-20ug/ml in formaldehyde fixed tissue. Reported to detect UCP2 in human neuroblastoma cell shy cells.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
U1621-12: Uncoupling Protein 2, Human, Control Peptide (UCP2, SLC25A8, UCPH)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.