Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)

Antibodies / Antibodies-Vitronectin

-20°C

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

Lysed bovine corneal endothelial cells and extracellular matrix.

Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75kD and 65kD forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:80, 000
Western Blot: 1:200
Immunohistochemistry: 1:50 using frozen, PLP-fixed sections of bovine tissues.
Optimal dilutions to be determined by the researcher.

Culture Medium:
RPMI 1640 with 10% fetal calf serum

Hybridoma:
Sp2/O myeloma cells with spleen cells from female BALB/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.