Comparison

Cy™3-conjugated AffiniPure® Goat Anti-Rat IgG, Light Chain* Specific (min X Bov,Gt,Hrs,Hu,Ms,Rb,Shp Ig)

Item no. 112-165-175
Manufacturer Jackson ImmunoResearch
Amount 0,5 mg
Category
Type Antibody Polyclonal
Format Lyophilized
Specific against Rat (Rattus norvegicus)
Host Caprine
Isotype IgG
Purity The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Specificity IgG, light chain specific
Manufacturer - Category
Anti‑Light Chain-Specific Antibodies for Western Blotting after IP
Manufacturer - Conjugate / Tag
Cyanine Cy™3
Storage Conditions
Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Expiration Date
one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Extended Storage after Rehydration
Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Preservative
0.05% Sodium Azide
Suggested Working Concentration or Dilution Range
1:100 - 1:800 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Buffer
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer
15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Manufacturer - Specificity
IgG, light chain specific
Clonality
Polyclonal
Minimal Cross Reactivity
Bovine, Goat, Horse, Human, Mouse, Rabbit, Sheep Immunoglobulins
Infomation
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on rat IgG and with those common to other rat immunoglobulins. Reaction is primarily with kappa light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Physical State
Freeze-dried solid
Purity
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0,5 mg
Available: In stock
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