Anti-proAKAP4 (clone 6F12)

Room temperature

12 months at -20°C
1 month at 4°C

Unconjugated

The measured concentration of the purified anti-proAKAP4 antibodies was 1 mg/mL as determined using a total protein concentration assay.

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.


This monoclonal antibody (clone 6F12) only recognizes the precursor/proform of AKAP4 (110 kDa / 854 AA), the prodomain (21 kDa) and does not react with the AKAP4 as a result of conversion (82 kDa / 665 AA).

Blommaert D, Sergeant N, Delehedde M, Jouy N, Mitchell V, Franck T, Donnay I, Lejeune JP and Serteyn D (2019b) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60.
- Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448
- Delehedde M, Carracedo S, Duchene B, Demouveaux B, Remy G, Kervoaze G, Aubry S, Gevaert MH, Maurage CA, Jonckheere N, Van Seuningen I, Philippe Gosset G, Desseyn JL, Sergeant N and Pichavant M (2020) Cigarette smoking affects the sperm-specific proAKAP4 concentrations and impairs both spermatogenesis and sperm quality. Andrology. Vol. 8 (Suppl. 1):45-46.
- Delehedde M, Carracedo S, Selleslagh M, Eddarkaoui S, Amirat-Briand L and Sergeant N (2019) ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. Vol. 2(1):13-19.
- Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019a) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86.
- Griffin R, Swegen A, Baker M, John Aitken R, A Skerrett-Byrne D, Silva Rodriguez A, E Martín-Cano F, Nixon B, J Peña F, Delehedde M, Sergeant N and Gibb Z (2020) Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 160(5):695-707.
- Langlade C, Buff S, Dias C, Commin L (2020) Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreserv Biobank. 18(5):409-414.
- Blommaert D, Franck T, Lejeune JF, Selleslagh M, Jouy N, Sergeant N, Serteyn D and Delehedde M (2019b) The Horse 4MID® Kit as a promising tool to compare different freezing media to preserve stallion semen. Rev. Bras. Reprod. Anim. Vol 43(2):585.
- Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. doi: 10.3390/biom10071046.
- Ruelle I, Sergeant N, Bencharif D, Charreaux F, Thorin C, Michaud S, Dordas-Perpinyà M, Jouy N, Audry S, Maurage CA, Delehedde M and Briand-Amirat L (2020) ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study. Reproduction, Fertility and Development. 32(1):145.
- Scovell JM, Bournat JC, Szafran AT, Solis M, Moore J, Rivera A, Chen CH, Zhang J, Wilken N, Seth A, Jorgez CJ (2021) PRSS50 is a testis protease responsible for proper sperm tail formation and function. Development Vol.148 (8): dev197558.
- Sergeant N, Blommaert D, Carracedo S, Franck T, Lejeune JP, Gevaert MH, Aubry S, Maurage CA, Serteyn D and Delehedde M (2020) Assessment of the proAKAP4 concentrations as a pertinent sperm parameter to select extenders and preservatives of stallion semen. Animal Reproduction Science. Vol. 220 :106449. P89. -
- Sergeant N, Carracedo S, Blommaert D, Aubry S, Jouy N, Lejeune JP, Franck T, Maurage CA, Serteyn D, Buée L and Delehedde M (2020) Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. Andrology. Vol. 8 (Suppl. 1):44-45.
- Delehedde M, Carracedo S, Selleslagh M, Eddarkaoui S, Amirat-Briand L and Sergeant N (2019) ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. Vol. 2(1):13-19.