Anti-WRN Picoband Antibody

WB

7486

Western blot analysis of WRN expression in rat thymus extract (lane 1) and human placenta extract (lane 2). WRN at 162KD, 200KD was detected using rabbit anti- WRN Antigen Affinity purified polyclonal antibody (Catalog # ABO11725) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .

0.1-0.5 µg/ml

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation. .

Werner syndrome ATP-dependent helicase

E. coli-derived human WRN recombinant protein (Position: Q122-N240). Human WRN shares 84% amino acid (aa) sequence identity with mouse WRN.

No cross reactivity with other proteins.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.