RAB27A Antibody

P51159

WB, FC

Seabra M.C., et al.J. Biol. Chem. 270:24420-24427(1995).
Seabra M.C., et al.Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
Chen D., et al.Biochem. Mol. Med. 60:27-37(1997).
Tolmachova T., et al.Gene 239:109-116(1999).
Hu R.-M., et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).

1590CT813.266.26

RAB27A

H

WB

Overlay histogram showing K562 cells stained with AM8511b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8511b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

24868

This RAB27A antibody is generated from a mouse immunized with a recombinant protein of human RAB27A.