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MITF Antibody

MITF Antibody

Item no.	ABC-AM8516b-ev
Manufacturer	Abcepta
Amount	50 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applications	WB
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	MITF, BHLHE32, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32
Available
Primary Accession	O75030
Antigen Type	Recombinant Protein
Application	WB
Bio Background	Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Bio References	Amae S., et al.Biochem. Biophys. Res. Commun. 247:710-715(1998). Tachibana M., et al.Hum. Mol. Genet. 3:553-557(1994). Wang Y., et al.BMC Med. 8:14-14(2010). Wiemann S., et al.Genome Res. 11:422-435(2001). Ota T., et al.Nat. Genet. 36:40-45(2004).
Clonality	monoclonal
Clone/Animal Names	1607CT834.207.47
Gene ID	4286
Gene Name	MITF
Subtitle	Purified Mouse Monoclonal Antibody (Mab)
Reactivity	H, M, Rat
Legend image 1	Immunohistochemical analysis of paraffin-embedded Human Melanoma section using Pink1(Cat#AM8516B). AM8516B was diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Type image 1	IHC
Dilution image 1	1:1000
Legend image 2	All lanes : Anti-MITF Antibody at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: PC-12 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Isotype	IgG1, k
Calculated MW	58795
Antigen Source	HUMAN
Target/Specificity	This MITF antibody is generated from a mouse immunized with a recombinant protein of human MITF.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 ul

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Delivery expected until 10/2/2025

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