Comparison

His Tag Antibody

Item no. ABC-AP1047a-ev-80ul
Manufacturer Abcepta
Amount 80 ul
Category
Type Antibody Polyclonal
Applications Western Blot
Specific against other
Host Rabbit
Isotype IgG
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Bio Background
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Bio References
Hochuli E, Doebeli H, and Schacher A. New metal chelate absorbent selective for proteins and peptides containing neighboring histidine residues. J. Chromatogr. 1987; 411:177-184.
Target/Specificity
Poly-HIS peptide were used to produced this antibody.

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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

 
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