PSMD3 Antibody (C-Term)

O43242

WB, FC, IHC-P

Kominami K., et al.Mol. Biol. Cell 8:171-187(1997).
Kalnine N., et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T., et al.Nat. Genet. 36:40-45(2004).
Zody M.C., et al.Nature 440:1045-1049(2006).
Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

RB55247

PSMD3

H, M

WB

AP22262b staining PSMD3 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

1:250

IHC-P

Overlay histogram showing HeLa cells stained with AP22262b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22262b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

B

60978

This PSMD3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 482-515 amino acids from human PSMD3.