Comparison

CD59 Antibody (Center)

Item no. ABC-AP22266c-ev
Manufacturer Abcepta
Amount 50 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC, IF, IHC-P
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products CD59, HRF-20, HRF20, MAC-IP, MACIF, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, 20 kDa homologous restriction factor, CD59 glycoprotein, 1F5 antigen, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin
Available
Primary Accession
P13987
Antigen Type
Synthesized Peptide
Application
WB, FC, IF, IHC-P-Leica
Bio Background
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
Bio References
Davies A., et al.J. Exp. Med. 170:637-654(1989).
Philbrick W.M., et al.Eur. J. Immunol. 20:87-92(1990).
Okada H., et al.Biochem. Biophys. Res. Commun. 162:1553-1559(1989).
Sugita Y., et al.J. Biochem. 106:555-557(1989).
Sawada R., et al.DNA Cell Biol. 9:213-220(1990).
Clonality
polyclonal
Clone/Animal Names
RB56691
Gene ID
966
Gene Name
CD59
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling CD59 with AP22266c at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Type image 1
IF
Dilution image 1
1:25
Legend image 2
All lanes : Anti-CD59 Antibody (Center) at 1:2000 dilution
Lane 1: HUVEC whole cell lysate
Lane 2: BxPC-3 whole cell lysate
Lane 3: PC-3 whole cell lysate
Lane 4: Human breast lysate
Lane 5: Human placenta lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 14 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2
WB
Dilution image 2
1:2000
Legend image 3
Immunohistochemical analysis of paraffin-embedded human placent tissue using AP22266c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 3
IHC-P-Leica
Dilution image 3
1:1000
Legend image 4
Immunohistochemical analysis of paraffin-embedded Human placenta section using Pink1(Cat#AP22266c). AP22266c was diluted at 1:250 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Type image 4
IHC
Dilution image 4
1:250
Legend image 5
Overlay histogram showing HeLa cells stained with AP22266c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22266c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 5
FC
Dilution image 5
1:25
Other Accession
Q28216
Isotype
Rabbit Ig
Calculated MW
14177
Antigen Source
HUMAN
Target/Specificity
This CD59 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 74-110 amino acids from the Central region of human CD59.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ul
Available: In stock
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