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Phospho-Bad(S99) Antibody

Phospho-Bad(S99) Antibody

Item no.	ABC-AP3039a
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Cell Biology Cell Status Apoptosis Immunohistochemistry Immunofluorescence Primary Antibodies Dot Blot
Type	Antibody Polyclonal
Applications	IF, IHC-P, Dot
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Citations	References for protein: 1.Hurbin, A., et al., J. Biol. Chem. 280(20):19757-19767 (2005). 2.Antignani, A., et al., Biochemistry 44(10):4074-4082 (2005). 3.Ying, S., et al., Infect. Immun. 73(3):1399-1403 (2005). 4.Seo, S.Y., et al., J. Biol. Chem. 279(40):42240-42249 (2004). 5.Lee, J.W., et al., Carcinogenesis 25(8):1371-1376 (2004). References for HeLa cell line: 1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695–710. [PubMed:13052828]. 2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141–50. [PubMed: 9892199]. 3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277–84. [PubMed: 19450234]. 4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1–8. [PubMed:20143388].
NCBI	NP_004313.1,NP_116784.1
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Bcl2-associated agonist of cell death,BAD,Bcl-2-binding component 6,Bcl-2-like protein 8,Bcl2-L-8,Bcl-xL/Bcl-2-associated death promoter,Bcl2 antagonist of cell death,BAD,BBC6,BCL2L8
Similar products	BAD, Bcl2 antagonist of cell death, Bcl-2-binding component 6, Bcl-xL/Bcl-2-associated death promoter, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-associated agonist of cell death, Bcl2-L-8
Available
Manufacturer - Category	Primary Antibodies; Antibody Collections; Cancer; Cell Biology; Metabolism; Signal Transduction; Amyotrophic Lateral Sclerosis (ALS); Phospho Antibodies; Apoptosis; Mitochondrion; Mitochondrion outer membrane
Manufacturer - Targets	This Bad Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.
Bio Background	Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.
Clonality	Polyclonal
Polyclonal Antibody Additonal Information	RB06933
Gene Name	BAD
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Predicted Reactivity	M, Rat
Calculated Molecular Weight	18392
Antigen Source	HUMAN
Formulation	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Dilution	IF: 1:200 IHC-P: 1:50~100 DB: 1:500
Antigen Type	Synthetic Peptide

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 1/1/2026

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