Comparison

TIM 3 Polyclonal Antibody

Item no. ABC-AP59082
Manufacturer Abcepta
Amount 100 ul
Category
Type Antibody Primary
Applications WB, IF, ICC, IHC-P, IHC-F
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Application
WB, IHC-P, IHC-F, IF, ICC
Clonality
Polyclonal
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
Rat
Legend image 1
Sample:
Thymus (Mouse) Lysate at 40 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-TIM 3 (bs-8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Type image 1
WB
Legend image 2
Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti-TIM 3 (bs-8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Type image 2
WB
Legend image 3
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-TIM 3 (bs-8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Type image 3
WB
Legend image 4
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min;
Incubation: Anti-TIM-3 Polyclonal Antibody, Unconjugated(bs-8766R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Type image 4
IHC-P

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ul
Available: In stock
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