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ABCB5 Antibody (N-term)

ABCB5 Antibody (N-term)

Item no.	ABC-AP6122a-ev
Manufacturer	Abcepta
Amount	80 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	ABCB5, ATP-binding cassette sub-family B member 5, ABCB5 P-gp, P-glycoprotein ABCB5
Available
Primary Accession	Q2M3G0
Antigen Type	Synthetic Peptide
Application	FC, WB
Bio Background	ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.
Bio References	Frank, N.Y., Cancer Res. 65 (10), 4320-4333 (2005) Chen, K.G., Pigment Cell Res. 18 (2), 102-112 (2005) Frank, N.Y., J. Biol. Chem. 278 (47), 47156-47165 (2003)
Clonality	Polyclonal
Clone/Animal Names	RB16781
Gene ID	340273
Gene Name	ABCB5 (HGNC:46)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-ABCB5 Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: A375 whole cell lysate Lane 5: A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Anti-ABCB5 Antibody (N-term) at 1:2000 dilution + Mouse eyeball lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	All lanes : Anti-ABCB5 Antibody (N-term) at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:2000
Legend image 4	Western blot analysis of ABCB5 Antibody (N-term) (Cat.#AP6122a) in A375, K562, A2058 and HL-60 cell line lysates (35ug/lane). ABCB5 (arrow) was detected using the purified Pab.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	Overlay histogram showing HepG2 cells stained with AP6122a(green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6122a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 5	FC
Dilution image 5	1:25
Legend image 6	Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# AP6122a on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
Type image 6	FC
Dilution image 6	1:10~50
Legend image 7	Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# AP6122a on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
Type image 7	FC
Dilution image 7	1:10~50
Isotype	Rabbit Ig
Calculated MW	138641
Antigen Region	1-30
Antigen Source	HUMAN
Target/Specificity	This ABCB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ABCB5.
NCBI Accession	NP_001157413.1; NP_001157414.1; NP_001157465.1; NP_848654.3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 80 ul

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Delivery expected until 9/4/2025

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