Comparison

WNT10B Antibody (Center)

Item no. ABC-AP6595b
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products WNT10B, Protein Wnt-10b, Protein Wnt-12, WNT12
Available
Primary Accession
O00744
Antigen Type
Synthetic Peptide
Application
WB, IHC-P, FC
Bio Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is a member of the WNT family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level.
Bio References
Zmuda, J.M., J. Bone Miner. Res. 24 (3), 437-447 (2009)
Pederson, L., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20764-20769 (2008)
Clonality
Polyclonal
Clone/Animal Names
RB19661
Gene ID
7480
Gene Name
WNT10B
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, M
Legend image 1
All lanes : Anti-WNT10B Antibody (Center) at 1:2000 dilution
Lane 1: Rat skeletal muscle whole lysate
Lane 2: Human skeletal muscle whole lysate


Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 43 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1
WB
Dilution image 1
1:2000
Legend image 2
WNT10B Antibody (Center) (Cat. #AP6595b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT10B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 2
IHC-P
Dilution image 2
1:10~50
Legend image 3
Flow cytometric analysis of MDA-231 cells using WNT10B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3
FC
Dilution image 3
1:10~50
Other Accession
P48614
Isotype
Rabbit Ig
Calculated MW
43000
Antigen Region
193-222
Antigen Source
HUMAN
Target/Specificity
This WNT10B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from the Central region of human WNT10B.
NCBI Accession
NP_003385.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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