VDAC1 Antibody (N-term)

Synthetic Peptide

VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

Polyclonal

7416

Purified Rabbit Polyclonal Antibody (Pab)

Anti-VDAC1 Antibody (N-term) at 1:8000 dilution + Raji whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 31 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:8000

WB

Western blot analysis of VDAC1 (arrow) using rabbit polyclonal VDAC1 Antibody (N-term) (Cat. #AP6627a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VDAC1 gene.

1:1000

IHC-P

VDAC1 Antibody (N-term) (Cat. #AP6627a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

B, Pig, Rb, Rat

30773

HUMAN

NP_003365.1