Comparison

MAPK12 Antibody (Center)

Item no. ABC-AP7224C
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications WB, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products MAPK12, ERK6, SAPK3, Mitogen-activated protein kinase 12, Extracellular signal-regulated kinase 6, Mitogen-activated protein kinase p38 gamma, Stress-activated protein kinase 3, MAP kinase 12, MAP kinase p38 gamma, MAPK 12, ERK-6
Available
Primary Accession
P53778
Antigen Type
Synthetic Peptide
Application
WB, IHC-P
Bio Background
Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. MAPK12 functions as a signal transducer during differentiation of myoblasts to myotubes.
Bio References
Garcia-Lora, A., et al., Cancer Immunol. Immunother. 52(1):59-64 (2003).
Julien, C., et al., J. Biol. Chem. 278(43):42615-42624 (2003).
Robinson, M.J., et al., J. Biol. Chem. 277(7):5094-5100 (2002).
Court, N.W., et al., J. Mol. Cell. Cardiol. 34(4):413-426 (2002).
Wang, X., et al., Mol. Cell. Biol. 20(13):4543-4552 (2000).
Clonality
Polyclonal
Clone/Animal Names
RB03195/03196
Gene ID
6300
Gene Name
MAPK12
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, M
Legend image 1
Western blot analysis of p38 gamma (MAPK12) Antibody (Center) (Cat.# AP7224c) in mouse lung tissue lysates (35ug/lane). MAPK12 (arrow) was detected using the purified Pab.
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
Western blot analysis of MAPK12 (arrow) using rabbit polyclonal hMAPK12-P354 (Cat. #AP7224c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
All lanes : Anti-MAPK12 Antibody (P354) at 1:2000 dilution
Lane 1: Hela whole cell lysates
Lane 2: Jurkat whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 42 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3
WB
Dilution image 3
1:2000
Legend image 4
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 4
IHC-P
Dilution image 4
1:50~100
Isotype
Rabbit Ig
Calculated MW
41940
Antigen Region
339-367
Antigen Source
HUMAN
Target/Specificity
This MAPK12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339-367 amino acids from the Central region of human MAPK12.
NCBI Accession
NP_002960.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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