SPHK1 Antibody (N-term)

Synthetic Peptide

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Polyclonal

8877

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of lysate from A431 cell line, using SPHK1 Antibody (M1)(Cat. #AP7237a). AP7237a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

1:1000

WB

Western blot analysis of anti-hSPHK1-M1 Pab (Cat. #AP7237a) in 293 cell line lysates transiently transfected with the SPHK1 gene (2ug/lane). hSPHK1-M1(arrow) was detected using the purified Pab.

1:1000

WB

Rabbit Ig

1-30

This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SPHK1.