NTF3 Antibody (C-term)

Synthetic Peptide

NTF3 is a member of the neurotrophin family that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.

Polyclonal

4908

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-NTF3 Antibody (C-term) at 1:2000 dilution
Lane 1: Human ovary lysate
Lane 2: Human spleen lysate


Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 29 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

All lanes : Anti-NTF3 Antibody (C-term) at 1:2000 dilution
Lane 1: Human liver lysate
Lane 2: Human ovary lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 29 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

Overlay histogram showing Hela cells stained with AP7763B(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP7763B, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

B, C, Pig, X

29355

HUMAN

NP_001096124.1; NP_002518.1