Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Isotype |
Ig |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, IHC-P |
Amount |
400 ul |
Host |
Rabbit |
Item no. |
ABC-AP7802b |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
P51617 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families. |
Bio References |
Jensen, L.E., et al., J. Immunol. 171(3):1500-1506 (2003).Jiang, Z., et al., J. Biol. Chem. 278(13):10952-10956 (2003).Cao, Z., et al., Science 271(5252):1128-1131 (1996).Strelow, A., et al., FEBS Lett. 547 (1-3), 157-161 (2003). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB02337 |
Gene ID |
3654 |
Gene Name |
|
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of anti-IRAK1 Pab (Cat. #AP7802b) in Jurkat cell lysate. IRAK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of IRAK1 (arrow) using rabbit polyclonal IRAK Antibody (C-term) (Cat. #AP802b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IRAK1 gene (Lane 2) (Origene Technologies). |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50~100 |
Legend image 4 |
Formalin-fixed and paraffin-embedded human Placenta tissue reacted with IRAK Antibody (C-term)(Cat.#AP7802b), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 4 |
IHC-P |
Dilution image 4 |
1:50~100 |
Legend image 5 |
Formalin-fixed and paraffin-embedded human Placenta tissue reacted with IRAK Antibody (C-term)(Cat.#AP7802b), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 5 |
IHC-P |
Dilution image 5 |
1:10~50 |
Isotype |
Rabbit Ig |
Calculated MW |
76537 |
Antigen Region |
683-712 |
Antigen Source |
HUMAN |
Target/Specificity |
This IRAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 683-712 amino acids from the C-terminal region of human IRAK. |
NCBI Accession |
NP_001020413.1; NP_001020414.1; NP_001560.2 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.