PIP5K1B Antibody (N-term)

Synthetic Peptide

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Polyclonal

8395

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-PIP5K1B Antibody (M1) at 1:1000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 61 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

WB

Formalin-fixed and paraffin-embedded human brain tissue reacted with PIP5K1B antibody (N-term) (Cat.# AP8038a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

1:50~100

Rabbit Ig

1-30

This PIP5K1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PIP5K1B.