HK2 (Hexokinase II) Antibody (Center)

Synthetic Peptide

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.

Polyclonal

3099

Purified Rabbit Polyclonal Antibody (Pab)

HK2 Antibody (R468) (Cat. #AP8140f) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow).

1:1000

IHC-P

Immunohistochemical analysis of paraffin-embedded H. stomach section using HK2 (Hexokinase II) Antibody (Center)(Cat#AP8140f). AP8140f was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

1:100

Rabbit Ig

453-483

This HK2 (Hexokinase II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-483 amino acids from the Central region of human HK2 (Hexokinase II).