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RB1 Antibody (C-term)

RB1 Antibody (C-term)

Item no.	ABC-AP8575B
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Rb, RB1, Retinoblastoma-associated protein, p105-Rb, pRb, pp110
Available
Primary Accession	P06400
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.
Bio References	Connell-Crowley, L., et.al., Mol. Biol. Cell 8 (2), 287-301 (1997) Kitagawa, M., et.al., EMBO J. 15 (24), 7060-7069 (1996)
Clonality	Polyclonal
Clone/Animal Names	RB20715
Gene ID	5925
Gene Name	RB1
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-RB1 Antibody (C-term) at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 106 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:8000
Legend image 2	Western blot analysis of RB1 Antibody (C-term) (Cat. #AP8575b) in 293 cell line lysates (35ug/lane). RB1 (arrow) was detected using the purified Pab.(2ug/ml)
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human testis tissue reacted with RB1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	RB1 Antibody (C-term) (Cat. #AP8575b) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	P33568
Predicted	Rat
Isotype	Rabbit Ig
Calculated MW	106159
Antigen Region	858-886
Antigen Source	HUMAN
Target/Specificity	This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 858-886 amino acids from the C-terminal region of human RB1.
NCBI Accession	NP_000312.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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