« Back
Home /
PTK2 Antibody (Center)

PTK2 Antibody (Center)

Item no.	ABC-AP8614c
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PTK2, FAK, Focal adhesion kinase 1, Focal adhesion kinase-related nonkinase, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FAK1, FRNK, PPP1R71, FADK 1, Protein phosphatase 1 regulatory subunit 71
Available
Primary Accession	Q05397
Antigen Type	Synthetic Peptide
Application	IF, FC, IHC-P, WB
Bio Background	PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.
Bio References	Calalb, M.B., et.al., Mol. Cell. Biol. 15 (2), 954-963 (1995) Polte, T.R. et.al., Proc. Natl. Acad. Sci. U.S.A. 92 (23), 10678-10682 (1995) Gervais, F.G., et.al., J. Biol. Chem. 273 (27), 17102-17108 (1998)
Clonality	Polyclonal
Clone/Animal Names	RB20775
Gene ID	5747
Gene Name	PTK2 (HGNC:9611)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of PTK2 Antibody (Center)(Cat#AP8614c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of PTK2 Antibody (Center) (Cat. #AP8614c) in T47D cell line lysates (35ug/lane). PTK2 (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of PTK2 (arrow) using rabbit polyclonal PTK2 Antibody (Center) (Cat. #AP8614c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK2 gene (Lane 2).
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:50~100
Legend image 5	Flow cytometric analysis of K562 cells using PTK2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 5	FC
Dilution image 5	1:10~50
Other Accession	O35346; Q00944; P34152
Predicted	C, M, Rat
Isotype	Rabbit Ig
Calculated MW	119233
Antigen Region	396-423
Antigen Source	HUMAN
Target/Specificity	This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the Central region of human PTK2.
NCBI Accession	NP_001186578.1; NP_722560.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ABC-AP8614c-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 400 ul

available

Delivery expected until 11/6/2025

Express Delivery: Save 14 days

Compare

Add to wishlist

Get an offer