Comparison

ATP1B2 Antibody (Center)

Item no. ABC-AP9271c
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Sodium/potassium-dependent ATPase subunit beta-2, AMOG, Adhesion molecule in glia
Available
Primary Accession
P14415
Antigen Type
Synthetic Peptide
Application
WB, FC, IHC-P
Bio Background
The protein belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Bio References
Guey, L.T., et.al., Eur. Urol. 57 (2), 283-292 (2010)
Tokhtaeva, E., et.al., Biochemistry 48 (48), 11421-11431 (2009)
Hosgood, H.D. et.al., Respir Med 103 (12), 1866-1870 (2009)
Clonality
Polyclonal
Clone/Animal Names
RB22612
Gene ID
482
Gene Name
ATP1B2
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Western blot analysis of lysates from K562 cell line and rat liver tissue lysate(from left to right), using ATP1B2 Antibody (Center)(Cat. #AP9271c). AP9271c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATP1B2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2
IHC-P
Dilution image 2
1:50~100
Legend image 3
ATP1B2 Antibody (Center) (Cat. #AP9271c) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3
FC
Dilution image 3
1:10~50
Other Accession
P13638; Q8WMG3; P14231; Q28030
Predicted
B, M, Rb, Rat
Isotype
Rabbit Ig
Calculated MW
33367
Antigen Region
115-141
Antigen Source
HUMAN
Target/Specificity
This ATP1B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-141 amino acids from the Central region of human ATP1B2.
NCBI Accession
NP_001669.3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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