UNG1 Antibody

P13051

UNG1 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promotes and alternative splicing. While UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from nuclear DNA that arise through either misincorporation during replication or cytosine deamination, inhibition of UNG1 by uracil glycosylase inhibitor did not lead to increased levels of spontaneous or induced mitochondrial DNA mutations. However, decreased levels of UNG activity and increased oxidative damage to mitochondrial DNA were seen in older mice, suggesting that mitochondrial DNA repair mechanisms may be involved in various neurodegenerative disorders in an age-dependent manner. This UNG1 antibody will not cross-react with UNG2.

Polyclonal

UNG {ECO:0000255|HAMAP-Rule:MF_03166}

Western blot analysis of UNG1 in C2C12 cell lysate with UNG1 antibody at (A) 0.5, (B) 1 and (C) 2 µg/mL.

Immunohistochemistry of UNG1 in human heart tissue with UNG1 antibody at 2 µg/mL.

Immunofluorescence of UNG1 in Human Heart cells with UNG1 antibody at 20 µg/mL.

NP_003353; 6224979

34645

UNG;

UNG1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

UNG1 antibody can be used for the detection of UNG1 by Western blot at 0.5 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 µg/mL. For immunofluorescence start at 20 µg/mL.

UNG1 Antibody is supplied in PBS containing 0.02% sodium azide.